WebTMPRSS2-ERG gene fusions are the predominant molecular subtype of prostate cancer. Here, we explored the role of TMPRSS2-ERG gene fusion product using in vitro and in vivo model systems. Transgenic mice expressing the ERG gene fusion product under androgen-regulation develop mouse prostatic intraepithelial neoplasia (PIN), a precursor lesion of ... Cell culture medium containing the final secreted protein product AA-(TMPRSS2(109–492))-EFVEHHHHHHH was collected by centrifugation (20 min, 10 °C, 6,000g) 4–5 d postinfection when cell viability dropped to 55–60%. Media was adjusted to pH 7.4 by addition of concentrated PBS stock, then … See more A construct encoding residues 109–492 comprising soluble TMPRSS2 ectodomain was amplified by two PCR fragments (Addgene plasmid no. 53887) and … See more Sf9 cells were grown in I-Max Insect Medium (Wisent Biocenter, catalog no. 301-045-LL) to a density of 4 × 106 cells per ml and infected with 20 ml l−1of suspension … See more Expi293F cells (Life Technologies catalog no. A1435102) were transiently transfected with expression plasmid encoding HexaPro/Furin using FectoPro … See more After size-exclusion purification of activated dasTMPRSS2, samples were pooled and concentrated to 2 mg ml−1. Protein was treated with 3:1 … See more
TMPRSS2 - an overview ScienceDirect Topics
WebJan 20, 2024 · Transmembrane serine protease 2 (TMPRSS2) is a cell surface protein primarily expressed by endothelial cells across the respiratory and digestive tracts. As a … WebMar 2, 2024 · We evaluated the stability of SARS-CoV-2 variants in aerosols and on high-density polyethylene (to represent a common surface) and estimated their decay rates by using a Bayesian regression model ().We generated aerosols (<5 μm) containing SARS-CoV-2 with a 3-jet Collison nebulizer and fed them into a Goldberg drum to create an … car and driver mercedes gle 450
7113 - Gene ResultTMPRSS2 transmembrane serine protease 2 [ (huma…
WebThe most common prostate cancer gene fusion, TMPRSS2-ERG, can be generated by the mechanism of interstitial deletion (Edel) about 39% to 60% of the time in clinically localized disease. Interestingly, we observed that all of the androgen-independent metastatic prostate cancer sites harboring TMPRSS2-ERG were associated with Edel. Web2024 coronavirus disease (COVID-19) is a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In addition to respiratory illness, COVID-19 patients exhibit neurological symptoms lasting from weeks to months (long COVID). It is unclear whether these neurological manifestati … WebOrder TMPRSS2 protein, a serine protease activating SARS-CoV-2 cell fusion. Purity 90%. Delivery in 1 week. Competive price at ProteoGenix. car and driver mid size suv